Some great tips, Adam. Thank you for all that.
Not excessive at all. There is a lot of mystery in mycology sometimes as to why things work or fail. I found myself doing the same, being excessively careful because I wanted to rule out or at least limit the likelihood of failure from things that might be preventable. We just about need a positive pressure clean room. One could possibly do that with a blower through a hepa filter but that would need to run continuously to keep contaminants out of the room. Oops, forgot about the HVAC . . . That would be a problem in that room. Maybe precool the house then shut down the A/C while you work.
While we are on the topic, here is something pretty cool with an insane amount of UV light output for sterilizing hospital rooms and ORs. Set the thing up, put a warning on the door and turn it on for about 20 minutes.
Reminds me of the UV germ lights near the ceiling in hospitals years ago. I used to take my UV erasable EPROMS over 30 years ago (2708s, etc) to the hospital to erase them. They were done in about 2 minutes under the lamp. I guess those are totally a thing of the past.
Good tips on leaving out the gloves on the box. Yes that could suck air in and out. Unless you had a 0.3 micron low resistance filtered air vent on the box and a great seal. One video shows the use of weather stripping along the box top. I think that if you repeatedly alcohol your hands then they achieve a really good level of sterility. Wash then alcohol then whenever you think about it reapply the alcohol.
A buddy of mine reported good results with something that he says was his own creation. He put some sort of small ‘air cleaner’ with HEPA filter onto the SAB to create a constant positive pressure / air flow. I forgot what he used but it was some sort of small ‘room air cleaner’ that would never have been effective at clearing a whole room but was more than enough for a SAB. He reported really low contamination. He somehow rigged a hose to the output. Wondering if one could find a cheap HEPA filtered vacuum cleaner and run the blower side into a box with a hose. Throttle the motor power a bit with a variac or electronic speed controller.
I see these at Costco on sale from time to time. They do move a lot of air and claim to have a sub micron filter. Might be good for general room air cleaning in a small, sealed, positive pressure room.