Dallas DIY BIO first Genetic Engineering Class successful bacteria transformation

quick video on first DNA Plasmid transfection

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This (free) class was taught by a UT Southwestern professor (PhD at MIT, 1 Post Doc at Cambridge) who is all about the Community Lab we’re building here at Dallas Makerspace! Usually, lab demos taught at universities are given by graduate students and not the professor… so I would argue that DMS is starting down a path of providing superior educational opportunities without the average 30K in debt.

@Robert_Davidson @lukeiamyourfather @Lampy @AlexRhodes @ESmith… just making sure y’all saw this

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Very cool mashup - if you ever do a version 2 could you somehow give the objectives of the experimentation? Just out of personal curiosity. Very cool stuff you’re doing!

Will do - we’ll always be working to improve our classes and accompanying materials and appreciate the feedback. Josh is the man when it comes to video!

To answer the question about the objective of this, this lab demonstrates incorporating a gene into bacteria that codes for green fluorescent protein (GFP). GFP occurs naturally in jellyfish and other marine organisms. When incorporated successfully, GFP causes the bacteria to glow under UV light demonstrating success! The pic below is from less than day old, burgeoning bacteria colonies.

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